Evaluation of pH-Sensitive Poly(β-amino ester)-graft-poly(ethylene glycol) and its Usefulness as a pH-Sensor and Protein Carrier

被引：10

作者：

Kim, Min Sang ^{[1
]}

Gao, Guang Hui ^{[1
]}

Kang, Seong Woo ^{[1
]}

Lee, Doo Sung ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Dept Polymer Sci & Engn, Suwon 440746, Gyeonggi, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 07期

关键词：

micelles; proteins; sensors; RESONANCE ENERGY-TRANSFER; BLOCK-COPOLYMER MICELLES; PYRENE; ESTER); POLYMERS; GENE; TRANSFECTION; DELIVERY; DRIVEN;

D O I：

10.1002/mabi.201100020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, some possible biomedical applications of a pH-sensitive and amphiphilic copolymer as a pH sensor and protein delivery system are reported. PAE-g-PEG was used as a pH-sensitive polymer that can exhibit a sharp pH-dependent transition. Various fluorescent dyes including pyrene and RITC can be used to label the pH-sensitive polymer PAE-g-PEG, which was evaluated for protein encapsulation. pH-sensing was possible by observing excimer formation of the labeled pyrene via pH-dependent expansion of the polymeric chain. Also, it was confirmed that FITC-BSA could be entrapped in RITC-labeled pH-sensitive micelles of PAE-g-PEG by FRET. As a result, PAE-g-PEG can be a pH sensor and carrier for protein delivery.

引用

页码：946 / 951

页数：6

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