High-resolution simultaneous voltage and Ca2+imaging

被引：30
|
作者：
Vogt, Kaspar E. [1 ]
Gerharz, Stephan [1 ]
Graham, Jeremy [2 ]
Canepari, Marco [3 ,4 ]
机构：
[1] Univ Basel, Biozentrum, Div Pharmacol & Neurobiol, CH-4003 Basel, Switzerland
[2] CAIRN Res Ltd, Faversham, England
[3] INSERM, Team 3, U836, F-38042 Grenoble 09, France
[4] Univ Grenoble 1, Inst Neurosci, F-38042 Grenoble 09, France
来源：
JOURNAL OF PHYSIOLOGY-LONDON | 2011年 / 589卷 / 03期
基金：
瑞士国家科学基金会;
关键词：
CALCIUM; TRANSIENTS; DENDRITES; CORTEX;
D O I：
10.1113/jphysiol.2010.200220
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Combining voltage and Ca2+ imaging allows the correlation of electrical and chemical activity at sub-cellular level. Here we describe a novel apparatus designed to obtain simultaneous voltage and Ca2+ measurements with single-trial resolution from sites as small as a few microns. These measurements can be obtained with negligible optical cross-talk between the two signals and negligible photo-damage of the preparation. The capability of the technique was assessed recording either from individual neurons in brain slices or from networks of cultured neurons. The present achievements open the gate to many novel physiological investigations requiring simultaneous measurement of voltage and Ca2+ signals.
引用
收藏
页码：489 / 494
页数：6
相关论文
共 50 条
  • [41] High-resolution ultrsound imaging and volume imaging
    Tranquart, F.
    JOURNAL DE RADIOLOGIE, 2006, 87 (12): : 1919 - 1919
  • [42] Characterizing enteric neuronal pathways activated by luminal nutrients using Ca2+imaging
    Fung, C.
    Hao, M.
    Boesmans, W.
    vanden Berghe, P.
    NEUROGASTROENTEROLOGY AND MOTILITY, 2018, 30
  • [43] Graphene Nanopore Support System for Simultaneous High-Resolution AFM Imaging and Conductance Measurements
    Connelly, Laura S.
    Meckes, Brian
    Larkin, Joseph
    Gillman, Alan L.
    Wanunu, Meni
    Lal, Ratnesh
    ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (07) : 5290 - 5296
  • [44] Development of High-Resolution Scanning Electrochemical Microscopy for Nanoscale Topography and Electrochemical Simultaneous Imaging
    Takahashi, Yasufumi
    ELECTROCHEMISTRY, 2016, 84 (09) : 662 - 666
  • [45] Simultaneous Polarization Measurement and High-Resolution Imaging Algorithm Inspired by Bat Sound Waveform
    Shen B.
    Liu T.
    Liu W.
    Gao G.
    IEEE Journal on Miniaturization for Air and Space Systems, 2022, 3 (04): : 264 - 275
  • [46] A High-resolution Multi-wavelength Simultaneous Imaging System with Solar Adaptive Optics
    Rao, Changhui
    Zhu, Lei
    Gu, Naiting
    Rao, Xuejun
    Zhang, Lanqiang
    Bao, Hua
    Kong, Lin
    Guo, Youming
    Zhong, Libo
    Ma, Xue'an
    Li, Mei
    Wang, Cheng
    Zhang, Xiaojun
    Fan, Xinlong
    Chen, Donghong
    Feng, Zhongyi
    Wang, Xiaoyun
    Wang, Zhiyong
    ASTRONOMICAL JOURNAL, 2017, 154 (04):
  • [47] The development of a high-resolution insert for simultaneous SPECT-MR imaging of small animals
    Tsui, Benjamin
    Xu, Jingyan
    Rittenbach, Andrew
    El-Sharkawy, Abd El-Monem
    Edelstein, William
    Liu, A.
    Parnham, Kevin
    Hugg, James
    JOURNAL OF NUCLEAR MEDICINE, 2012, 53
  • [48] Bioinspired design of synthetic polymer-based Ca2+sensor for the realization of extracellular Ca2+imaging
    Ishiwari, Fumitaka
    Fukushima, Takanori
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 254
  • [49] SYSTEM FOR SIMULTANEOUS HIGH-RESOLUTION MARINE PROFILING
    HOUGH, JC
    GEOPHYSICS, 1977, 42 (07) : 1509 - 1509
  • [50] Ca2+Imaging of pacemaker activity in interstitial cells of Cajal in human jejunum in situ
    Lee, Hyun-Tai
    Hennig, Grant W.
    Fleming, Neal
    Ward, Scan M.
    Keef, Kathleen D.
    Spencer, Nick J.
    Sanders, Kenton M.
    Smith, Terence K.
    GASTROENTEROLOGY, 2006, 130 (04) : A378 - A378
12345