A new method for the detection and elimination of Hydrangea ringspot virus

被引：2
|
作者：
Toth, E. K. [4 ]
Kriston, E. [2 ,3 ]
Mathe, A. [1 ]
Jambor-Benczur, E. [5 ]
机构：
[1] Univ W Hungary, Fac Agr & Food Sci, H-9200 Mosonmagyarovar, Hungary
[2] Directorate Plant Protect, Agr Off, H-1118 Budapest, Hungary
[3] Agrienvironm Labs Pest Diag, H-1118 Budapest, Hungary
[4] Lab Plant Pathol & Biotechnol, H-1039 Budapest, Hungary
[5] Corvinus Univ Budapest, Dept Ornamental Plants & Dendrol, Fac Hort Sci, H-1118 Budapest, Hungary
来源：
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY | 2012年 / 87卷 / 01期
关键词：
INHIBITION; GROWTH;
D O I：
10.1080/14620316.2012.11512823
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The complete eradication of Hydrangea ringspot virus (HRSV) from Hydrangea by in vitro pre-treatment with ribavirin and meristem tip culture is reported. A sensitive RT-PCR method for the detection of HRSV in newly-formed regenerants was developed. Total nucleic acid extracts from HRSV-infected Hydrangea leaves diluted 10(5)-fold gave a positive reaction using this method.
引用
收藏
页码：13 / 16
页数：4
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