Clinicogenetic investigations in iron-deficiency anemia.

To analyse clinicogenetic associations in iron-deficiency anemia (IDA) and to assess informative value of genetic markers with validation of their value in predicting predisposition to the disease, we have examined 102 IDA patients and 105 healthy subjects (control group). Polymerase chain reaction of DNA synthesis in thermocycler working in automatic regime and using locus-specific oligonucleotide primers was applied for analysis of polymorphic loci of xenobiotics detoxication genes: cytochrome P-4501A1 (CYP1A1), glutathione S-transferase M1 (GSTM1), N-acetyltransferase 2 (Nat2), ACE, plasminogen activator (PA). High incidence of a mutant form of gene CYP1A1 and homozygous deletion of PA gene in IDA patients vs controls was found as well as combinations of genotypes by polymorphic systems CYP1A1, GSTM1, Nat2, ACE and PA characteristic for IDA patients which can be used as molecular-genetic markers of predisposition to the disease.