Portable Benzo[a]pyrene Immunosensor Based on the Polyanline Nanofiber Modified Screen-printed Electrode

被引:2
|
作者
Wang, Chunping [1 ,2 ]
Zhou, Jiedan [1 ]
Huang, Yibin [1 ]
Liang, Genquan [1 ]
Liu, Yingju [1 ]
Lei, Hongtao [2 ]
机构
[1] South China Agr Univ, Coll Sci, Inst Biomat, Guangzhou 510642, Guangdong, Peoples R China
[2] South China Agr Univ, Higher Educat Key Lab Food Quality & Safety, Guangzhou 510642, Guangdong, Peoples R China
来源
关键词
Immunosensor; Nanofiber; Screen-printed electrode; Benzo[a]pyrene; POLYCYCLIC AROMATIC-HYDROCARBONS; EXTRACTION; HPLC;
D O I
10.4028/www.scientific.net/AMR.221.295
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
In this work, polyaniline nanofiber was synthesized. Then, polyaniline nanofiber and chitosan were dropped onto the surface of the self-prepared screen-printed electrode (SPE), respectively. After aminopyrene-BSA conjugate was immobilized on the modified electrode surface with the aid of EDC/NHS solution, the primary antibody was dropped on the modified SPE and followed by the HRP-labeled secondary antibody, thus a disposable immunosensor was developed for the rapid detection of benzo[a]pyrene based on an indirect immunoassay.
引用
收藏
页码:295 / +
页数:2
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