A new tool for probing of cell-cell communication: human embryonic germ cells inducing apoptosis of SKOV3 ovarian cancer cells on a microfluidic chip

被引：13

作者：

Song, Xinyan ^{[1
]}

Kong, Beihua ^{[1
]}

Li, Dong ^{[2
]}

机构：

[1] Shandong Univ, Dept Obstet & Gynecol, Qilu Hosp, Jinan 250012, Peoples R China

[2] Shandong Univ, Cryomed Lab, Qilu Hosp, Jinan 250012, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 09期

基金：

中国国家自然科学基金;

关键词：

apoptosis; embryonic germ cells; microfluidic chip; ovarian cancer;

D O I：

10.1007/s10529-008-9725-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A microfluidic device with unidirectional perfusion has been developed to observe the effect of human embryonic germ (hEG) cells on SKOV3 cells. The hEG and SKOV3 cells were seeded in the inlet and the outlet reservoirs separately, and co-cultured for 2 days. The medium was perfused unidirectionally from the inlet to the outlet. The growth inhibition of SKOV3 cells was monitored online and the apoptosis signals in SKOV3 culture area decreased along the flow of the medium. In conclusion, microfluidic chip is a potentially useful tool to investigate the effect of stem cells on cancer cells with intuitionistic cell-based screens.

引用

页码：1537 / 1543

页数：7

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