Potentiometric biosensors for cholinesterase inhibitor analysis based on mediatorless bioelectrocatalysis

被引：38
|
作者：
Ghindilis, AL [1 ]
Morzunova, TG [1 ]
Barmin, AV [1 ]
Kurochkin, IN [1 ]
机构：
[1] RES CTR MOL DIAGNOST & THERAPY,DEPT BIOSENSORS,MOSCOW 113149,RUSSIA
来源：
BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 09期
关键词：
mediatorless bioelectrocatalysis; butyrylcholinesterase; choline oxidase; peroxidase; inhibitor cholinesterase; biosensor;
D O I：
10.1016/0956-5663(96)89436-X
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A potentiometric method for cholinesterase inhibitor analysis based on mediatorless bioelectrocatalysis has been developed. The method includes coimmobilization of three enzymes, butyrylcholinesterase, choline oxidase and peroxidase, on composite carbon electrodes. Catalytic hydrolysis of butyrylcholine and subsequent catalytic oxidation of choline result in the formation of hydrogen peroxide leads to a shift in the electrode potential. The detection limit for trichlorfon analysis is 2 x 10(-13) M. Electrodes remain stable for at least 4 weeks when stored at 277 K. (C) 1996 Elsevier Science Limited.
引用
收藏
页码：873 / 880
页数：8
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