Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics

被引：23

作者：

Schellhaus, Anna Katharina ^{[1
,2
]}

Moreno-Andres, Daniel ^{[1
,2
]}

Chugh, Mayank ^{[3
]}

Yokoyama, Hideki ^{[1
,2
]}

Moschopoulou, Athina ^{[1
]}

De, Suman ^{[3
]}

Bono, Fulvia ^{[4
]}

Hipp, Katharina ^{[4
]}

Schaeffer, Erik ^{[3
]}

Antonin, Wolfram ^{[1
,2
]}

机构：

[1] Max Planck Gesell, Friedrich Miescher Lab, Spemannstr 39, D-72076 Tubingen, Germany

[2] Rhein Westfal TH Aachen, Inst Biochem & Mol Cell Biol, Sch Med, D-52074 Aachen, Germany

[3] Univ Tubingen, Ctr Plant Mol Biol ZMBP, Cellular Nanosci, D-72076 Tubingen, Germany

[4] Max Planck Inst Dev Biol, Spemannstr 35, D-72076 Tubingen, Germany

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

GENE; END; IDENTIFICATION; COMPLEX; FAMILY; CHROMATIN; TUBULIN; CLONING; CANCER;

D O I：

10.1038/s41598-017-10088-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mitotic spindle, essential for segregating the sister chromatids into the two evolving daughter cells, is composed of highly dynamic cytoskeletal filaments, the microtubules. The dynamics of microtubules are regulated by numerous microtubule associated proteins. We identify here Developmentally regulated GTP binding protein 1 (DRG1) as a microtubule binding protein with diverse microtubule-associated functions. In vitro, DRG1 can diffuse on microtubules, promote their polymerization, drive microtubule formation into bundles, and stabilize microtubules. HeLa cells with reduced DRG1 levels show delayed progression from prophase to anaphase because spindle formation is slowed down. To perform its microtubule-associated functions, DRG1, although being a GTPase, does not require GTP hydrolysis. However, all domains are required as truncated versions show none of the mentioned activities besides microtubule binding.

引用

页数：16

共 50 条

[31] Association of a novel GTP binding protein, DRG, with TAL oncogenic proteins
Mahajan, MA
Park, ST
Sun, XH
[J]. ONCOGENE, 1996, 12 (11) : 2343 - 2350
[32] Assignment of the gene for a developmentally regulated GTP-binding protein (DRG2) to human chromosome bands 17p13→p12 by in situ hybridization
Schenker, T
Trueb, B
[J]. CYTOGENETICS AND CELL GENETICS, 1997, 79 (3-4): : 274 - 275
[33] DYNAMIN - A MICROTUBULE-ASSOCIATED GTP-BINDING PROTEIN
OBAR, RA
SHPETNER, HS
VALLEE, RB
[J]. JOURNAL OF CELL SCIENCE, 1991, : 143 - 145
[34] A developmentally regulated GTP binding tyrosine phosphorylated protein A-like cDNA in cucumber (Cucumis sativus L.)
Matat Barak
Tova Trebitsh
[J]. Plant Molecular Biology, 2007, 65 : 829 - 837
[35] Characterization of DRGs, developmentally regulated GTP-binding proteins, from pea and Arabidopsis
Michelle L. Devitt
Kenneth J. Maas
Joel P. Stafstrom
[J]. Plant Molecular Biology, 1999, 39 : 75 - 82
[36] Developmentally regulated GTP-binding protein 2 ameliorates EAE by suppressing the development of TH17 cells
Ko, Myoung Seok
Kim, Hyo Jeong
Kim, Hong Kyung
Yoon, Nal Ae
Lee, Unn Hwa
Lee, Sang Chul
Chung, Dae Kyun
Lee, Byung Ju
Suh, Jae Hee
Cho, Wha Ja
Park, Jeong Woo
[J]. CLINICAL IMMUNOLOGY, 2014, 150 (02) : 225 - 235
[37] Characterization of DRGs, developmentally regulated GTP-binding proteins, from pea and Arabidopsis
Devitt, ML
Maas, KJ
Stafstrom, JP
[J]. PLANT MOLECULAR BIOLOGY, 1999, 39 (01) : 75 - 82
[38] A developmentally regulated GTP binding tyrosine phosphorylated protein A-like cDNA in cucumber (Cucumis sativus L.)
Barak, Matat
Trebitsh, Tova
[J]. PLANT MOLECULAR BIOLOGY, 2007, 65 (06) : 829 - 837
[39] Characterization of DRGs, developmentally regulated GTP binding proteins, from pea and Arabidopsis.
Maas, K
Devitt, M
Stafstrom, J
[J]. PLANT PHYSIOLOGY, 1997, 114 (03) : 1496 - 1496
[40] Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine
Michael Prattes
Irina Grishkovskaya
Victor-Valentin Hodirnau
Ingrid Rössler
Isabella Klein
Christina Hetzmannseder
Gertrude Zisser
Christian C. Gruber
Karl Gruber
David Haselbach
Helmut Bergler
[J]. Nature Communications, 12

← 1 2 3 4 5 →