Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

被引：37

作者：

Wang, Zhihua ^{[1
]}

Wu, Wenjun ^{[1
]}

Wang, Zhuo ^{[1
]}

Tang, Ying ^{[1
]}

Deng, Yuliang ^{[1
]}

Xu, Ling ^{[2
]}

Tian, Jianhui ^{[2
]}

Shi, Qihui ^{[1
,3
,4
]}

机构：

[1] Shanghai Jiao Tong Univ, Minist Educ, Key Lab Syst Biomed, Shanghai, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Shanghai, Peoples R China

[3] Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Shanghai, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China

来源：

ANALYST | 2016年 / 141卷 / 12期

基金：

中国国家自然科学基金;

关键词：

BREAST-CANCER CELLS; CHIP; CULTURE; CAPTURE; 2-NBDG;

D O I：

10.1039/c5an02554k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels.

引用

页码：3621 / 3625

页数：5

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