Dynamic molecular imaging of living cells by using Raman scattering

被引：0
|
作者：
Fujita, Katsumasa [1 ]
Hamada, Keisaku [2 ]
Smith, Nicholas Isaac [1 ]
Inouye, Yasushi [2 ]
Kawata, Satoshi [1 ,3 ]
机构：
[1] Osaka Univ, Dept Appl Phys, 2-1 Yamadaoka, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Front Biosci, Suita, Osaka 5650871, Japan
[3] RIKEN, Wako, Saitama 3510198, Japan
来源：
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VII | 2009年 / 7182卷
关键词：
Raman scattering; molecular imaging; live cell imaging; confocal microscopy; FLUORESCENCE; CYTOCHROME; SPECTRA;
D O I：
10.1117/12.809053
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Raman spectroscopy has been utilized to investigate properties of biomolecules due to its capability of detecting molecular vibrations that represent molecular species, structures and environmental conditions. In this research, we used Raman scattering to image dynamics of molecules in living cells. By combining the 532 nm excitation and a slit-scanning detection technique, we observed dynamics of molecular distributions of cytochrome, protein beta sheets, and lipids in an unstained HeLa cell during mitosis with a frame rate of 5 minutes.
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页数：5
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