Protocol for Initiating and Monitoring Bumble Bee Microcolonies with Bombus impatiens (Hymenoptera: Apidae)

被引：0
|
作者：
Lehmann, David M. [1 ]
机构：
[1] US EPA, Ctr Publ Hlth & Environm Assessment CPHEA, Hlth & Environm Effects Assessment Div, Integrated Hlth Assessment Branch, Res Triangle Pk, NC 27711 USA
来源：
BIO-PROTOCOL | 2022年 / 12卷 / 12期
关键词：
Bumble bee; Bombus; Microcolony; Pollinators; Pesticides; IMPACT; POLLEN;
D O I：
10.21769/BioProtoc.4451
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Populations of some bumble bee species are in decline, prompting the need to better understand bumble bee biology and for assessing the effects of environmental stressors on these important pollinators. Microcolonies have been successfully used for investigating a range of endpoints, including behavior, gut microbiome, nutrition, development, pathogens, and the effects of pesticide exposure on bumble bee health. Here, we present a step-by-step protocol for initiating, maintaining, and monitoring microcolonies with Bombus impatiens. This protocol has been successfully used in two pesticide exposure-effects studies and can be easily expanded to investigate other aspects of bumble bee biology.
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页数：14
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