Multimodal Multiphoton Microscope with Depth Scanning

被引：3

作者：

Wu, Wentao ^{[1
]}

Liu, Qihao ^{[1
]}

Brandt, Christoph ^{[1
]}

Tang, Shuo ^{[1
]}

机构：

[1] Univ British Columbia, Dept Elect & Comp Engn, 5500-2332 Main Mall, Vancouver, BC V6T 1Z4, Canada

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XXII | 2022年 / 11965卷

关键词：

multiphoton; MPM; multimodal; depth-scanning; SMA; FLUORESCENCE; 2-PHOTON; CELLS;

D O I：

10.1117/12.2608365

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. An Er-doped fiber laser with 1580 nm and 790 nm output provides the dual-wavelength multimodal capability and a shape-memory-alloy (SMA) based depth-scanning objective enables the depth scanning. Image stacks combining two-photon-excitation-fluorescence (TPEF), second-harmonic-generation (SHG), and third-harmonic-generation (THG) signals have been acquired on animal samples from the surface to over 200 mu m underneath.

引用

页数：6

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