Multimodal Multiphoton Microscope with Depth Scanning

被引：3
|
作者：
Wu, Wentao [1 ]
Liu, Qihao [1 ]
Brandt, Christoph [1 ]
Tang, Shuo [1 ]
机构：
[1] Univ British Columbia, Dept Elect & Comp Engn, 5500-2332 Main Mall, Vancouver, BC V6T 1Z4, Canada
来源：
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XXII | 2022年 / 11965卷
关键词：
multiphoton; MPM; multimodal; depth-scanning; SMA; FLUORESCENCE; 2-PHOTON; CELLS;
D O I：
10.1117/12.2608365
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. An Er-doped fiber laser with 1580 nm and 790 nm output provides the dual-wavelength multimodal capability and a shape-memory-alloy (SMA) based depth-scanning objective enables the depth scanning. Image stacks combining two-photon-excitation-fluorescence (TPEF), second-harmonic-generation (SHG), and third-harmonic-generation (THG) signals have been acquired on animal samples from the surface to over 200 mu m underneath.
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页数：6
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