A sterol biosynthetic pathway in Mycobacterium

被引：54
|
作者：
Lamb, DC
Kelly, DE
Manning, NJ
Kelly, SL [1 ]
机构：
[1] Univ Wales, Inst Biol Sci, Aberystwyth SY23 3DA, Dyfed, Wales
[2] Sheffield Childrens Hosp, Sheffield S10 2UH, S Yorkshire, England
来源：
FEBS LETTERS | 1998年 / 437卷 / 1-2期
关键词：
sterol; biosynthesis; inhibition; Mycobacterium;
D O I：
10.1016/S0014-5793(98)01218-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The genome sequence of Mycobacterium tuberculosis (and also M. leprae) revealed a significant number of homologies to Saccharomyces cerevisiae sterol biosynthetic enzymes. We addressed the hypothesis of a potential sterol biosynthetic pathway existing in Mycobacterium using cultures of Mycobacterum smegmatis. Non-saponifiable lipid extracts subjected to analysis by gas chromatography-mass spectrometry (GC-MS) showed cholesterol was present. Sterol synthesis by M. smegmatis was confirmed using C-14-radiolabelled mevalonic acid and incorporation into C4-desmethyl sterol co-migrating with authentic cholesterol on TLC. The sterol biosynthetic pathway has provided a rich source of targets for commercially important bioactive molecules and such agents represent new opportunities for Mycobacteria ia chemotherapy. (C) 1998 Federation of European Biochemical Societies.
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页码：142 / 144
页数：3
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