Detection of chrysotile asbestos by using a chrysotile-binding protein

被引：23
|
作者：
Kuroda, Akio [1 ,2 ]
Nishimura, Tomoki [1 ]
Ishida, Takenori [2 ]
Hirota, Ryuichi [1 ]
Nomura, Kazutaka [1 ]
机构：
[1] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, Hiroshima 7398530, Japan
[2] Japan Sci & Technol Corp, SORST, Kawaguchi, Saitama 3320012, Japan
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 2008年 / 99卷 / 02期
关键词：
asbestos; detection; chrysotile; chrysotile-binding protein; alkaline phosphatase; environmental issue;
D O I：
10.1002/bit.21588
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In the current studies, we found that the DksA protein from Escherichia coli binds strongly to chrysotile, which is the most commonly used form of asbestos. We developed a convenient colorimetric assay for chrysotile using a fusion of DksA and alkaline phosphatase along with 5-bromo-4-chloro-3-indolyi-phosphate and nitro blue tetrazolium as substrates. Also, using a fusion of DksA and green-fluorescent protein, we were able to detect chrysotile by fluorescence microscopy.
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页码：285 / 289
页数：5
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