Engineering molecular recognition in alkane oxidation catalysed by cytochrome P450cam

被引：20
|
作者：
Stevenson, JA [1 ]
Bearpark, JK [1 ]
Wong, LL [1 ]
机构：
[1] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England
来源：
NEW JOURNAL OF CHEMISTRY | 1998年 / 22卷 / 06期
关键词：
D O I：
10.1039/a801637b
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The activity, selectivity and coupling efficiency of alkane oxidation by the monooxygenase enzyme cytochrome P450(cam) can be enhanced by engineering the active site topology to accommodate different alkanes: reducing the active site volume by the Y96F-V247L double mutations resulted in four-fold higher activity for the oxidation of hexane (1) than 3-methylpentane (2) while the larger active site of the Y96A-V247A double mutant gave rise to a two-fold preference for 2 over 1.
引用
收藏
页码：551 / 552
页数：2
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