4Pi-confocal microscopy provides three-dimensional images of the microtubule network with 100- to 150-nm resolution

被引：55
|
作者：
Nagorni, M [1 ]
Hell, SW [1 ]
机构：
[1] Max Planck Inst Biophys Chem, High Resolut Opt Microscopy Grp, D-37070 Gottingen, Germany
来源：
JOURNAL OF STRUCTURAL BIOLOGY | 1998年 / 123卷 / 03期
关键词：
D O I：
10.1006/jsbi.1998.4037
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We show the applicability of 4Pi-confocal microscopy to three-dimensional imaging of the microtubule network in a fixed mouse fibroblast cell. Comparison with two-photon confocal resolution reveals a fourfold better axial resolution in the 4Pi-confocal case. By combining 4Pi-confocal microscopy with Richardson-Lucy image restoration a further resolution increase is achieved. Featuring a three-dimensional resolution in the range 100-150 nm, the 4Pi-confocal (restored) images are intrinsically more detailed than their confocal counterparts. Our images constitute what to our knowledge are the best-resolved three-dimensional images of entangled cellular microtubules obtained with light to date. (C) 1998 Academic Press.
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页码：236 / 247
页数：12
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