Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples

被引:0
|
作者
Manuel F Juette
Travis J Gould
Mark D Lessard
Michael J Mlodzianoski
Bhupendra S Nagpure
Brian T Bennett
Samuel T Hess
Joerg Bewersdorf
机构
[1] Institute for Molecular Biophysics,Department of Biophysical Chemistry, and Department of New Materials and Biosystems
[2] The Jackson Laboratory,Department of Physics and Astronomy
[3] University of Heidelberg,undefined
[4] Max Planck Institute for Metals Research,undefined
[5] University of Maine,undefined
[6] Institute for Molecular Biophysics,undefined
[7] University of Maine,undefined
[8] Active Motif,undefined
[9] Inc.,undefined
来源
Nature Methods | 2008年 / 5卷
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摘要
The ability to image thick volumes with invariant high axial and lateral resolution is a challenge for existing super-resolution fluorescence microscopy techniques. The combination of a double-plane detection scheme with fluorescence photoactivation microscopy (FPALM) allows three-dimensional sub-diffraction resolution imaging of samples as thick as whole cells.
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页码:527 / 529
页数:2
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