The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration

被引：147

作者：

McGonigle, Louise ^{[1
]}

Mousley, Angela ^{[1
]}

Marks, Nikki J. ^{[1
]}

Brennan, Gerard P. ^{[1
]}

Dalton, John P. ^{[2
]}

Spithill, Terry W. ^{[3
,4
]}

Day, Tim A. ^{[5
]}

Maule, Aaron G. ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Biol Sci, Ctr Med Biol, Belfast BT9 7BL, Antrim, North Ireland

[2] Univ Technol Sydney, Inst Biotechnol Infect Dis, Sydney, NSW 2007, Australia

[3] McGill Univ, Ctr Host Parasite Interactions, Inst Parasitol, FQRNT, Montreal, PQ H3A 2T5, Canada

[4] Charles Sturt Univ, Sch Agr & Vet Sci, Wagga Wagga, NSW 2678, Australia

[5] Iowa State Univ, Dept Biomed Sci, Program Neurosci, Ames, IA 50011 USA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2008年 / 38卷 / 02期

关键词：

Fasciola; newly excysted juvenile; cathepsin L; cathepsin B; RNA interference; tissue penetration;

D O I：

10.1016/j.ijpara.2007.10.007

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Probing protein function in parasitic flatworms is hampered by the difficulties associated with the development of transgenic approaches. Although RNA interference (RNAi) in schistosomes shows much promise, it has not been reported in other trematodes. Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs). Silencing resulted in marked reductions in target transcript levels and significant diminution in the encoded proteins in the gut. RNAi of either enzyme in NEJs induced transient, abnormal locomotory phenotypes, and significantly reduced penetration of the rat intestinal wall. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：149 / 155

页数：7