Y-chromosomal STR systems: Applications of a triplex PCR in forensic stain analysis

The analysis of polymorphic short tandem repeats (STR) provides the opportunity of rapid identification of forensic stains. In cases of mixed stains of male and female DNA, Y-chromosomal STR-systems can be used to analyse male DNA without having to cope with high background of the victim's female DNA. We established a tripler PCR including the three Y-chromosomal STR-systems DYS 393, DYS 19 and DYS 392 and tested its applicability in forensic stain analysis and determined population data for the population in Cologne.