Simultaneous determination of ketamine and xylazine in canine plasma by liquid chromatography with ultraviolet absorbance detection

被引：29
|
作者：
Niedorf, F [1 ]
Bohr, HH [1 ]
Kietzmann, M [1 ]
机构：
[1] Tierarztlichen Hsch Hannover, Inst Pharmacol Toxicol & Pharm, D-30559 Hannover, Germany
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 791卷 / 1-2期
关键词：
ketamine; xylazine;
D O I：
10.1016/S1570-0232(03)00215-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of ketamine and xylazine in canine plasma is described. Plasma samples (500 mul) are cleaned up via liquid-liquid extraction. The analytes and the internal standard clonidine are separated on a cyano (CN) column using a mobile phase containing acetonitrile-0.005 M phosphate buffer adjusted to pH 5.5 (3:2) at a detection wavelength of 215 run. The method was validated according to specificity, sensitivity, accuracy and reproducibility and was used to determine the plasma concentrations of both compounds in dogs after intramuscular injection. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：421 / 426
页数：6
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