Monitoring protein ubiquitination and SUMOylation in real-time by NMR

被引：2
|
作者：
Habibullah, Batul Ismail [1 ]
Tripathi, Vasvi [1 ]
Surana, Parag [1 ]
Das, Ranabir [1 ]
机构：
[1] Natl Ctr Biol Sci, Bengaluru 560065, India
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 49期
关键词：
14;
D O I：
10.1039/d0cc02252g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.
引用
收藏
页码：6735 / 6738
页数：4
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