Biophysical measurements of cells, microtubules, and DNA with an atomic force microscope

被引:6
|
作者
Devenica, Luka M. [1 ]
Contee, Clay [1 ]
Cabrejo, Raysa [1 ]
Kurek, Matthew [1 ]
Deveney, Edward F. [2 ]
Carter, Ashley R. [1 ]
机构
[1] Amherst Coll, Dept Phys, Amherst, MA 01002 USA
[2] Bridgewater State Univ, Dept Phys, Bridgewater, MA 02325 USA
基金
美国国家科学基金会;
关键词
AFM; REGISTRATION; STABILITY; MOLECULES; FILAMENTS;
D O I
10.1119/1.4941048
中图分类号
G40 [教育学];
学科分类号
040101 ; 120403 ;
摘要
Atomic force microscopes (AFMs) are ubiquitous in research laboratories and have recently been priced for use in teaching laboratories. Here, we review several AFM platforms and describe various biophysical experiments that could be done in the teaching laboratory using these instruments. In particular, we focus on experiments that image biological materials (cells, microtubules, and DNA) and quantify biophysical parameters including membrane tension, persistence length, contour length, and the drag force. (C) 2016 American Association of Physics Teachers.
引用
收藏
页码:301 / 310
页数:10
相关论文
共 50 条
  • [41] Density measurements of single granules using the atomic force microscope
    Andersson, KM
    Bergström, L
    JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 2005, 88 (08) : 2322 - 2324
  • [42] Friction, adhesion, and deformation: dynamic measurements with the atomic force microscope
    Attard, P
    JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, 2002, 16 (07) : 753 - 791
  • [43] Localized surface elasticity measurements using an atomic force microscope
    DeVecchio, D
    Bhushan, B
    REVIEW OF SCIENTIFIC INSTRUMENTS, 1997, 68 (12): : 4498 - 4505
  • [44] USE OF ATOMIC-FORCE MICROSCOPE FOR THE MEASUREMENTS OF HYDROPHOBIC FORCES
    RABINOVICH, YI
    YOON, RH
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1994, 93 : 263 - 273
  • [45] Friction, adhesion, and deformation: Dynamic measurements with the atomic force microscope
    Attard, P. (phil.attard@unisa.edu.au), 1600, Taylor and Francis Ltd. (16):
  • [46] Measurements of magnetic effects on electrolyte solutions by atomic force microscope
    Higashitani, K
    Oshitani, J
    PROCESS SAFETY AND ENVIRONMENTAL PROTECTION, 1997, 75 (B2) : 115 - 119
  • [47] Uncertainty quantification in nanomechanical measurements using the atomic force microscope
    Wagner, Ryan
    Moon, Robert
    Pratt, Jon
    Shaw, Gordon
    Raman, Arvind
    NANOTECHNOLOGY, 2011, 22 (45)
  • [48] Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells
    Severac, Childerick
    Proa-Coronado, Sergio
    Formosa-Dague, Cecile
    Martinez-Rivas, Adrian
    Dague, Etienne
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021, (170):
  • [49] ATOMIC-FORCE MICROSCOPE MEASUREMENTS OF NUCLEOSOME CORES ASSEMBLED ALONG DEFINED DNA-SEQUENCES
    ALLEN, MJ
    DONG, XF
    ONEILL, TE
    YAU, P
    KOWALCZYKOWSKI, SC
    GATEWOOD, J
    BALHORN, R
    BRADBURY, EM
    BIOCHEMISTRY, 1993, 32 (33) : 8390 - 8396
  • [50] Atomic force microscope
    不详
    MICRO, 1995, 13 (10): : 22 - 22