α-Glucosidase Inhibitory Mechanism of Phloridzin

被引：0
|
作者：
Liu, Jiangwei [1 ]
Chen, Yuzhen [2 ]
Liu, Benguo [1 ]
Liang, Guizhao [3 ]
机构：
[1] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Peoples R China
[2] Henan Inst Sci & Technol, Sch Math Sci, Xinxiang 453003, Peoples R China
[3] Chongqing Univ, Sch Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China
来源：
JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN | 2018年 / 40卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Phloridzin; alpha-Glucosidase; Inhibitory mechanism; Fluorescence spectroscopy; Molecular docking; MOLECULAR DOCKING; FLAVONOIDS; IDENTIFICATION; ANTIOXIDANT; DERIVATIVES; AMYLASE; 3D-QSAR; TRYPSIN; SEARCH;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Phloridzin is a chalcone with potential application in functional food and medicine. In this study, the alpha-glucosidase inhibitory mechanism of phloridzin was investigated by inhibitory test, fluorescence spectroscopy and molecular docking method. The IC50 value of phloridzin was determined at 0.68 mmol/L. With the increase of phloridzin concentration, the static fluorescence quenching of alpha-glucosidase could be observed. The quenching constant (Kq), binding constant (K-A) and the number of binding site (n) of phloridzin to alpha-glucosidase were 6.1474x10(12), 1.3243X10(5) and 1.076, respectively. The molecular docking result suggested that phloridzin could play the inhibitory role by binding the Lys510 and G1u374 of alpha-glucosidase with hydrogen bonds.
引用
收藏
页码：799 / 803
页数：5
相关论文
共 50 条
  • [1] Inhibitory kinetics and mechanism of kaempferol on α-glucosidase
    Peng, Xi
    Zhang, Guowen
    Liao, Yijing
    Gong, Deming
    FOOD CHEMISTRY, 2016, 190 : 207 - 215
  • [2] Inhibitory Mechanism of Isoliquiritigenin against α-Glucosidase
    Han, Fenxia
    Fan, Xinjing
    Geng, Sheng
    Lou, Wenjuan
    Liang, Guizhao
    Liu, Benguo
    Shipin Kexue/Food Science, 2019, 40 (15): : 37 - 42
  • [3] Inhibitory Mechanism of Engeletin Against α-Glucosidase
    Li, Yunbo
    Liu, Xiaoling
    Zhou, Haoyu
    Li, Bo
    Mazurenko, Igor Kostiantinovich
    NATURAL PRODUCT COMMUNICATIONS, 2021, 16 (01)
  • [4] Inhibitory Mechanism of Baicalein Against α-Glucosidase
    He, Chengyun
    Liu, Xiaoling
    Sun, Junliang
    NATURAL PRODUCT COMMUNICATIONS, 2019, 14 (06)
  • [5] Laccase Catalyzed Oxidative Polymerization of Phloridzin: Polymer Characterization, Antioxidant Capacity and α-Glucosidase Inhibitory Activity
    Zhou, Haoyu
    Liu, Changzhong
    Geng, Sheng
    NATURAL PRODUCT COMMUNICATIONS, 2021, 16 (10)
  • [6] Inhibitory mechanism of apigenin, quercetin, and phloretin on α-glucosidase
    Ren, Xin
    Liu, Xuan
    Wang, Bu zhen
    Zhang, Min
    Wang, Lin xuan
    FOOD BIOSCIENCE, 2024, 62
  • [7] Inhibitory kinetics and mechanism of oleanolic acid on α-glucosidase
    Zhike Xie
    Ming He
    Yuhan Zhai
    Feifei Xin
    Shuyan Yu
    Shaoxuan Yu
    Haifang Xiao
    Yuanda Song
    Journal of Food Measurement and Characterization, 2021, 15 : 3408 - 3418
  • [8] Inhibitory kinetics and mechanism of oleanolic acid on α-glucosidase
    Xie, Zhike
    He, Ming
    Zhai, Yuhan
    Xin, Feifei
    Yu, Shuyan
    Yu, Shaoxuan
    Xiao, Haifang
    Song, Yuanda
    JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2021, 15 (04) : 3408 - 3418
  • [9] THE MECHANISM OF PHLORIDZIN GLUCOSURIA
    SHAPIRO, B
    BIOCHEMICAL JOURNAL, 1947, 41 (02) : 151 - 154
  • [10] Mechanism for the Inhibitory Effect of 1-Deoxynojirimycin on α-Glucosidase
    Guo, Shiyin
    Li, Lin
    Zhou, Hong
    Tang, Zhonghai
    Su, Xiaojun
    Li, Qingming
    Shipin Kexue/Food Science, 2019, 40 (05): : 45 - 50
12345