Gene transduction by sonoporation

被引：0

作者：

Ohta, Sho ^{[2
]}

Suzuki, Kentaro ^{[1
]}

Ogino, Yukiko ^{[1
]}

Miyagawa, Shinichi ^{[1
]}

Murashima, Aki ^{[1
]}

Matsumaru, Daisuke ^{[1
]}

Yamada, Gen ^{[1
]}

机构：

[1] Kumamoto Univ, Grad Sch Med & Pharmaceut Sci, CARD, Kumamoto 8608611, Japan

[2] Univ Utah, Sch Med, Dept Neurobiol & Anat, Salt Lake City, UT USA

来源：

DEVELOPMENT GROWTH & DIFFERENTIATION | 2008年 / 50卷 / 06期

关键词：

gene transduction chick; limb bud; ultrasound; microbubble; sonoporation;

D O I：

10.1111/j.1440-169x.2008.01026.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.

引用

页码：517 / 520

页数：4

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