The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor β-subunit

被引：26
|
作者：
del Pino, Isabel [1 ]
Paarmann, Ingo [1 ]
Karas, Michael [2 ]
Kilimann, Manfred W. [3 ]
Betz, Heinrich [1 ]
机构：
[1] Max Planck Inst Brain Res, Dept Neurochem, D-60528 Frankfurt, Germany
[2] Goethe Univ Frankfurt, Inst Pharmaceut Chem, D-60438 Frankfurt, Germany
[3] Uppsala Univ, Dept Neurosci, S-75124 Uppsala, Sweden
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 412卷 / 03期
关键词：
Glycine receptor; Spinal cord; Gephyrin; Neurobeachin; Retromer complex; Vacuolar Protein Sorting 35; CHEDIAK-HIGASHI-SYNDROME; SPLICE VARIANTS; GEPHYRIN; ACTIVATION; RETROMER; IDENTIFICATION; COMPLEXES; ENDOSOME; KINASE; VPS29;
D O I：
10.1016/j.bbrc.2011.07.110
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Inhibitory glycine receptors (GlyRs) are densely packed in the postsynaptic membrane due to a high-affinity interaction of their beta-subunits with the scaffolding protein gephyrin. Here, we used an affinity-based proteomic approach to identify the trafficking proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea) as novel GlyR beta-subunit (GlyR beta) interacting proteins in rat brain. Recombinant Vps35 and a central fragment of Nbea bound to the large intracellular loop of GlyR beta in glutathione-S-transferase pull-downs; in addition, Vps35 displayed binding to gephyrin. Immunocytochemical staining of spinal cord sections revealed Nbea immunoreactivity apposed to and colocalizing with marker proteins of inhibitory synapses. Our data are consistent with roles of Vps35 and Nbea in the retrieval and post-Golgi trafficking of synaptic GlyRs and possibly other neurotransmitter receptors. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：435 / 440
页数：6
相关论文
共 50 条
  • [31] The Dyggve Melchior Clausen syndrome protein, dymeclin interacts with vacuolar protein sorting protein 25 supporting its role in endoplasmic reticulum trafficking
    Kinning, Esther
    Shackleton, S.
    Trembath, R. C.
    JOURNAL OF MEDICAL GENETICS, 2006, 43 : S28 - S28
  • [32] Golgi-mediated vacuolar sorting in plant cells: RMR proteins are sorting receptors for the protein aggregation/membrane internalization pathway
    Park, Joon Ho
    Oufattole, Mohammed
    Rogers, John C.
    PLANT SCIENCE, 2007, 172 (04) : 728 - 745
  • [33] Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35
    Mingmin Yan1
    NeuralRegenerationResearch, 2012, 7 (09) : 652 - 658
  • [34] Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35
    Yan, Mingmin
    Mao, Shanping
    Dong, Huimin
    Liu, Baohui
    Zhang, Qian
    Pan, Gaofeng
    Fu, Zhiping
    NEURAL REGENERATION RESEARCH, 2012, 7 (09) : 652 - 658
  • [35] Endocytic trafficking of the neuropeptideY receptor 2 & interaction with G protein-coupled receptor associated sorting protein 1
    Jolly, Richard
    Hislop, James
    Thompson, Dawn
    BRITISH JOURNAL OF PHARMACOLOGY, 2023, 180 : 689 - 690
  • [36] Retraction Note to: Full recovery of the Alzheimer’s disease phenotype by gain of function of vacuolar protein sorting 35
    Jian-Guo Li
    Jin Chiu
    Domenico Praticò
    Molecular Psychiatry, 2023, 28 : 965 - 965
  • [37] RETRACTED ARTICLE: Full recovery of the Alzheimer’s disease phenotype by gain of function of vacuolar protein sorting 35
    Jian-Guo Li
    Jin Chiu
    Domenico Praticò
    Molecular Psychiatry, 2020, 25 : 2630 - 2640
  • [38] The plant vacuolar sorting receptor AtELP is involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana
    Ahmed, SU
    Rojo, E
    Kovaleva, V
    Venkataraman, S
    Dombrowski, JE
    Matsuoka, K
    Raikhel, NV
    JOURNAL OF CELL BIOLOGY, 2000, 149 (07): : 1335 - 1344
  • [39] Arabidopsis TOL Proteins Act as Gatekeepers for Vacuolar Sorting of PIN2 Plasma Membrane Protein
    Korbei, Barbara
    Moulinier-Anzola, Jeanette
    De-Araujo, Lucinda
    Lucyshyn, Doris
    Retzer, Katarzyna
    Khan, Muhammad A.
    Luschnig, Christian
    CURRENT BIOLOGY, 2013, 23 (24) : 2500 - 2505
  • [40] PROTEIN SORTING IN YEAST - MUTANTS DEFECTIVE IN VACUOLE BIOGENESIS MISLOCALIZE VACUOLAR PROTEINS INTO THE LATE SECRETORY PATHWAY
    ROTHMAN, JH
    STEVENS, TH
    CELL, 1986, 47 (06) : 1041 - 1051
12345