Medium design based on stoichiometric analysis of microbial transglutaminase production by Streptoverticillium mobaraense

被引：7

作者：

Zhu, Y ^{[1
]}

Rinzema, A ^{[1
]}

Tramper, J ^{[1
]}

Bol, J ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR,DEPT FOOD SCI,FOOD & BIOPROC ENGN GRP,6700 EV WAGENINGEN,NETHERLANDS

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1996年 / 50卷 / 03期

关键词：

stoichiometric model; medium design; microbial transglutaminase;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A stoichiometric model was developed for the application of medium design in microbial transglutaminase production by Streptoverticillium mobaraense. The model avoids dealing with all the metabolic reactions involved by simply lumping them into a single reaction. With the help of measurement results, an ana lysis of the nutrients' roles, and biochemical knowledge of the microorganism, all stoichiometric coefficients in the model were calculated. These coefficients were used for medium design. With this designed medium, microbial transglutaminase activity was increased fourfold, compared to that in the basal medium. (C) 1996 John Wiley & Sons, Inc.

引用

页码：291 / 298

页数：8

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