Importance of the characteristics of the chromatographic separation in HPLC for the interpretation of HbA1c assay in the presence of a variant hemoglobin

被引：1
|
作者：
Guillard, Emmanuelle [1 ]
Jaisson, Stephane [1 ]
Gillery, Philippe [1 ]
机构：
[1] CHU Reims, Lab Biol & Rech Pediat, Reims, France
来源：
ANNALES DE BIOLOGIE CLINIQUE | 2010年 / 68卷 / 05期
关键词：
HPLC; HbA(1c); hemoglobinopathy; PRESSURE LIQUID-CHROMATOGRAPHY; GLYCATED HEMOGLOBIN; ANALYZER;
D O I：
10.1684/abc.2010.0472
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
The methods of HbA(1c) assay using ion exchange high pressure liquid chromatography (HPLC) allow the detection of the most common hemoglobin variants. This observation highlights the different behaviour of two HPLC analyzers in the presence of Tatras hemoglobin. By one of the analyzer (Variant II, Bio-Rad) this variant is detected, but not by the other (G8, Tosoh Biosciences). As HbA(1c) result is crucial for the therapeutic decision, it is important that biologists know the characteristics of the method they use, in order to detect the possible occurence of an hemoglobinopathy and to ensure the best interpretation of the result.
引用
收藏
页码：598 / 602
页数：5
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