The Lower Cretaceous Abu Gabra formation is the main source rock of the Muglad basin oils. It has been previously believed that this source rock consists of a single organofacies. This study reveals that at least two different organofacies units exist. A detailed biomarker study of a suite of 57 oil samples was carried out using Gas chromatographic (GC) and Gas Chromatographic/Mass Spectrometric (GCMS) data. Two main oil families ("Family-A" and "Family-B") have been identified based on this. Distinction between these was solely based on variations in Pristane/Phytane (Pr/Ph) ratios, Diahopane indices, Diasterane/Regular sterane ratios, and Regular steranes distributions. "Family-A" being basically restricted to oils from Heglig, Unity, and Bamboo fields, tend to show relatively low (Pr/Ph) ratios in the range of (1.3 to 1.9), low Diahopane indices (4.1-9.6), low diasterane/regular sterane ratio (0.34-0.60), and relatively high C-28 Regular sterane content (24-32). In contrast, "Family-B" that is typified by the remaining oils, displays a relatively high (Pr/Ph) ratios (1.23 to 2.27), high Diahopane indices (10.8-32.0), high diasterane/regular sterane ratio (0.52-1.30), and relatively low C28 Regular sterane content (23-28). The facies unit that generated "Family-A" oils is more algal (high C28 Steranes), and was deposited in a more closed, less oxic high-stand system (low Pr/Ph, Diahopane index, and Diasterane/regular sterane) typical of early rift phase lacustrine environments. "Family-B" oil seems to have originated from a more terriginous suite (higher Pr/Ph, low C-28 Regular Sterane), and was deposited in a more open, oxic-sub-oxic, shallow-stand system (high Diahopane index, diasterane/regular sterane), that is a late rift phase environment.