Transcriptomic-based bioassays for the detection of type A trichothecenes

被引:10
|
作者
Lancova, K. [1 ]
Bowens, P. [1 ]
Stroka, J. [2 ]
Gmuender, H. [3 ]
Ellinger, T. [4 ]
Naegeli, H. [1 ]
机构
[1] Univ Zurich Vetsuisse, Inst Pharmacol & Toxicol, CH-8057 Zurich, Switzerland
[2] Commiss European Communities, Joint Res Ctr, Food Safety & Qual Unit, Inst Reference Mat & Measurements, B-2440 Geel, Belgium
[3] Genedata AG, CH-4016 Basel, Switzerland
[4] Clondiag GmbH, D-07749 Jena, Germany
关键词
chemokines; cytokines; microarray; real-time PCR; mitogen-activated protein kinases; ACTIVATED PROTEIN-KINASE; RIBOTOXIC STRESS-RESPONSE; T-2; TOXIN; MICROARRAY ANALYSIS; DEOXYNIVALENOL; MYCOTOXINS; EXPRESSION; FOOD; INHIBITION; CEREALS;
D O I
10.3920/WMJ2008.1125
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The type A trichothecenes T-2 toxin (T-2) and HT-2 toxin (HT-2) are hazardous Fusarium products that contaminate many field crops growing in cold to temperate regions across the world. Toxicity studies in laboratory and farm animals have been used to derive a temporary tolerable daily intake (t-TDI) for the sum of T-2 and HT-2 of no more than 60 ng/kg body weight. To protect the consumers, it is now necessary to screen a large number of food samples for the presence of these poisonous fungal metabolites. Towards that goal, we discovered that the transcriptional apparatus of a human carcinoma cell line (MCF7) provides a sensitive biological sensor of type A trichothecenes. In fact, exposure of this easy-to-culture cell line to T-2 or HT-2 results in the regulation of >2,000 different transcripts with expression changes ranging from >5,000-fold gene inductions to >40-fold gene repressions. These transcriptional responses have been exploited to develop practical microchip and reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of type A trichothecenes at parts per billion levels.
引用
收藏
页码:247 / 257
页数:11
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