DEVELOPMENT AND CHARACTERIZATION OF POLYMORPHIC MICROSATELLITE MARKERS FOR CONOPHOLIS AMERICANA (OROBANCHACEAE)

被引：2
|
作者：
Rodrigues, Anuar G. [1 ]
Colwell, Alison E. L. [2 ]
Stefanovic, Sasa [1 ]
机构：
[1] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada
[2] Div Biol Resources, El Portal, CA 95318 USA
来源：
AMERICAN JOURNAL OF BOTANY | 2012年 / 99卷 / 01期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Conopholis americana; microsatellite; Orobanchaceae; parasitic plants; WINDOWS; LINUX;
D O I：
10.3732/ajb.1100269
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Conopholis americana is an obligate root parasite with highly reduced morphology. To investigate the population structure, genetic diversity, and mating system of this predominantly eastern North American species, we developed polymorphic microsatellite markers for C. americana. Methods and Results: Using an enrichment cloning protocol, we report the isolation and characterization of 11 microsatellite markers. Product size varied from 198-370 bp. These loci show moderate levels of allelic variation (averaging 4.182 alleles per locus) and very low levels of heterozygosity (average observed heterozygosity = 0.054). Conclusions: These microsatellite markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. americana.
引用
收藏
页码：E4 / E6
页数：3
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