Isolation of DNA aptamers using micro free flow electrophoresis

被引：68
|
作者：
Jing, Meng [1 ]
Bowser, Michael T. [1 ]
机构：
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55414 USA
来源：
LAB ON A CHIP | 2011年 / 11卷 / 21期
基金：
美国国家卫生研究院;
关键词：
IN-VITRO SELECTION; PROBE CAPILLARY-ELECTROPHORESIS; MOLECULAR RECOGNITION; RNA-APTAMERS; THERAPEUTIC APPLICATIONS; AFFINITY-CHROMATOGRAPHY; MICROMAGNETIC SELECTION; EXPONENTIAL ENRICHMENT; REVERSE-TRANSCRIPTASE; SYSTEMATIC EVOLUTION;
D O I：
10.1039/c1lc20461k
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A micro free flow electrophoresis (mu FFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of mu FFE allowed 1.8 x 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of mu FFE selection.
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页码：3703 / 3709
页数：7
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