Asiaticoside attenuates lipopolysaccharide-induced acute lung injury via down-regulation of NF-κB signaling pathway

被引:55
|
作者
Qiu, Jiaming [1 ,2 ]
Yu, Lijun [3 ]
Zhang, Xingxing [2 ]
Wu, Qianchao [2 ]
Wang, Di [3 ]
Wang, Xiuzhi [3 ]
Xia, Cheng [1 ]
Feng, Haihua [2 ]
机构
[1] Heilongiang Bayi Agr Univ, Key Lab Anim Med, Daqing, Peoples R China
[2] Jilin Univ, Coll Vet Med, Key Lab Zoonosis, Minist Educ, Changchun 130062, Jilin, Peoples R China
[3] Inner Mongolia Univ Nationalities, Inst Med Chem & Pharmacol, Tongliao 028000, Inner Mongolia, Peoples R China
关键词
Asiaticoside; ALI; LPS; Cytokines; NF-kappa B; RESPIRATORY-DISTRESS SYNDROME; ALVEOLAR FLUID CLEARANCE; ACTIVATED RAW264.7 CELLS; EPITHELIAL-CELLS; SEPTIC SHOCK; CYTOKINES; SEPSIS; MACROPHAGES; INHIBITION; EXPRESSION;
D O I
10.1016/j.intimp.2015.03.022
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Asiaticoside (AS), a triterpene glycoside isolated from Centella asiatica, has been shown to possess potent anti-inflammatory activity. However, the detailed molecular mechanisms of AS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in mice are scanty. The purpose of this study was to evaluate the effect of AS on LPS-induced mouse ALL via down-regulation of NF-kappa B signaling pathway. We investigated the efficacy of AS on cytokine levels induced by LPS in bronchoalveolar lavage fluid (BALF) and RAW 264.7 cells. The production of cytokine (TNF-alpha and IL-6) was measured by enzyme-linked immunosorbent assay (ELISA). The lung wet-to-dry weight ratios were measured in LPS-challenged mice, and lung histopathologic changes observed via paraffin section were assessed. To further study the mechanism of AS protective effects on ALI, the activation of NF-kappa B p65 subunit and the degradation of I kappa B alpha were tested by western blot assay. We found that AS treatment at 15,30 or 45 mg/kg dose-dependently attenuated LPS-induced pulmonary inflammation by reducing inflammatory infiltration, histopathological changes, descended cytokine production, and pulmonary edema initiated by LPS. Furthermore, our results suggested that AS suppressed inflammatory responses in LPS-induced ALI through inhibition of the phosphorylation of NF-kappa B p65 subunit and the degradation of its inhibitor I kappa B alpha, and might be a new preventive agent of ALI in the clinical setting. (c) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:181 / 187
页数:7
相关论文
共 50 条
  • [1] Remifentanil Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Downregulating the NF-κB Signaling Pathway
    Zhang, Ying
    Du, Zhaohui
    Zhou, Qing
    Wang, Yanlin
    Li, Jianguo
    [J]. INFLAMMATION, 2014, 37 (05) : 1654 - 1660
  • [2] Remifentanil Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Downregulating the NF-κB Signaling Pathway
    Ying Zhang
    Zhaohui Du
    Qing Zhou
    Yanlin Wang
    Jianguo Li
    [J]. Inflammation, 2014, 37 : 1654 - 1660
  • [3] Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway
    Li, Weishi
    Fu, Kaiqiang
    Lv, Xiaopei
    Wang, Yu
    Wang, Jifang
    Li, Huatao
    Tian, Wenru
    Cao, Rongfeng
    [J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2015, 28 (01) : 695 - 699
  • [4] Cavidine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via NF-κB Signaling Pathway in vivo and in vitro
    Niu, Xiaofeng
    Liu, Fang
    Li, Weifeng
    Zhi, Wenbing
    Zhang, Hailin
    Wang, Xiumei
    He, Zehong
    [J]. INFLAMMATION, 2017, 40 (04) : 1111 - 1122
  • [5] Cavidine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via NF-κB Signaling Pathway in vivo and in vitro
    Xiaofeng Niu
    Fang Liu
    Weifeng Li
    Wenbing Zhi
    Hailin Zhang
    Xiumei Wang
    Zehong He
    [J]. Inflammation, 2017, 40 : 1111 - 1122
  • [6] Alkannin attenuates lipopolysaccharide-induced lung injury in mice via Rho/ROCK/NF-κB pathway
    Li, Bin
    Lin, Qingling
    Hou, Qiliang
    Yin, Chao
    Zhang, Lei
    Li, Yumin
    [J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2019, 33 (07)
  • [7] Tetrahydroberberrubine attenuates lipopolysaccharide-induced acute lung injury by down-regulating MAPK, AKT, and NF-κB signaling pathways
    Yu, Xiu
    Yu, Sulan
    Chen, Ling
    Liu, Han
    Zhang, Jian
    Ge, Haixia
    Zhang, Yuanyuan
    Yu, Boyang
    Kou, Junping
    [J]. BIOMEDICINE & PHARMACOTHERAPY, 2016, 82 : 489 - 497
  • [8] Acanthopanax senticosus attenuates inflammation in lipopolysaccharide-induced acute lung injury by inhibiting the NF-κB pathway
    Fei, X. J.
    Zhu, L. L.
    Xia, L. M.
    Peng, W. B.
    Wang, Q.
    [J]. GENETICS AND MOLECULAR RESEARCH, 2014, 13 (04): : 10537 - 10544
  • [9] Echinacoside attenuates lipopolysaccharide-induced acute lung injury in newborn mice via inactivation of NF-κB/NLRP3 signaling pathway
    Fu, Meijiao
    Shen, Tong
    Yang, Ying
    Zheng, Yaling
    Zhong, Lilin
    [J]. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, 2020, 19 (09) : 1815 - 1819
  • [10] Baicalin Magnesium Salt Attenuates Lipopolysaccharide-Induced Acute Lung Injury via Inhibiting of TLR4/NF-κB Signaling Pathway
    Zhang, Lin
    Yang, Lukun
    Xie, Xiaowei
    Zheng, Hongyue
    Zheng, Hangsheng
    Zhang, Lizong
    Liu, Cuizhe
    Piao, Ji-Gang
    Li, Fanzhu
    [J]. JOURNAL OF IMMUNOLOGY RESEARCH, 2021, 2021