Cavidine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via NF-κB Signaling Pathway in vivo and in vitro

被引:40
|
作者
Niu, Xiaofeng [1 ]
Liu, Fang [1 ]
Li, Weifeng [1 ]
Zhi, Wenbing [1 ]
Zhang, Hailin [1 ]
Wang, Xiumei [1 ]
He, Zehong [1 ]
机构
[1] Xi An Jiao Tong Univ, Sch Pharm, 76 Western Yanta Rd, Xian 710061, Shaanxi Provinc, Peoples R China
关键词
ALI; Cavidine; LPS; NF-kappa B p65; I kappa B alpha; RESPIRATORY-DISTRESS-SYNDROME; MICE; INFLAMMATION; INHIBITION; EXPRESSION; PROTEIN; MODEL; SUPPRESSION; MODULATION; ACTIVATION;
D O I
10.1007/s10753-017-0553-1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Acute lung injury (ALI) is characterized by widespread inflammation in the lungs and alveolar-capillary destruction, causing high morbidity and mortality. Cavidine, isolated from Corydalis impatiens, have been exhibited to have potent anti-inflammatory effects in previous studies. The purpose of this study was to evaluate the protective effect of cavidine on lipopolysaccharide (LPS)-induced ALI and to enunciate the underlying in vivo and in vitro mechanisms. Mice were intraperitoneally administrated with cavidine (1, 3, or 10 mg/kg) at 1 and 12 h, prior to the induction of ALI by intranasal administration of LPS (30 mg/kg). Blood samples, lung tissues, and bronchoalveolar lavage fluid (BALF) were harvested after LPS challenge. Furthermore, we used LPS-induced lung epithelial cells A549 to examine the mechanism of cavidine to lung injury. The results showed that pretreatment with cavidine significantly decreased lung wet-to-dry weight (W/D) ratio, reduced pro-inflammatory cytokine levels including TNF-alpha and IL-6 in BALF and serum from LPS-stimulated mice, and attenuated lung histopathological changes. In addition, western blot results showed that cavidine inhibited the phosphorylation of nuclear factor-kappaB (NF-kappa B) p65 and I kappa B alpha induced by LPS. In conclusion, our results demonstrate that cavidine protects against LPS-induced acute lung injury in mice via inhibiting of pro-inflammatory cytokine TNF-alpha and IL-6 production and NF-kappa B signaling pathway activation. Taken together, cavidine may be useful for the prevention and treatment of pulmonary inflammatory diseases, such as ALI.
引用
收藏
页码:1111 / 1122
页数:12
相关论文
共 50 条
  • [1] Cavidine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via NF-κB Signaling Pathway in vivo and in vitro
    Xiaofeng Niu
    Fang Liu
    Weifeng Li
    Wenbing Zhi
    Hailin Zhang
    Xiumei Wang
    Zehong He
    [J]. Inflammation, 2017, 40 : 1111 - 1122
  • [2] Polydeoxyribonucleotide ameliorates lipopolysaccharide-induced acute lung injury via modulation of the MAPK/NF-κB signaling pathway in rats
    Ko, Il-Gyu
    Hwang, Jae Joon
    Chang, Bok Soon
    Kim, Sang-Hoon
    Jin, Jun-Jang
    Hwang, Lakkyong
    Kim, Chang-Ju
    Choi, Cheon Woong
    [J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2020, 83
  • [3] Piperine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Modulating NF-κB Signaling Pathways
    Lu, Ying
    Liu, Jingyao
    Li, Hongyan
    Gu, Lina
    [J]. INFLAMMATION, 2016, 39 (01) : 303 - 308
  • [4] Piperine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Modulating NF-κB Signaling Pathways
    Ying Lu
    Jingyao Liu
    Hongyan Li
    Lina Gu
    [J]. Inflammation, 2016, 39 : 303 - 308
  • [5] Effects of acteoside on lipopolysaccharide-induced inflammation in acute lung injury via regulation of NF-κB pathway in vivo and in vitro
    Wang Jing
    Ma Chunhua
    Wang Shumin
    [J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2015, 285 (02) : 128 - 135
  • [6] Remifentanil Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Downregulating the NF-κB Signaling Pathway
    Zhang, Ying
    Du, Zhaohui
    Zhou, Qing
    Wang, Yanlin
    Li, Jianguo
    [J]. INFLAMMATION, 2014, 37 (05) : 1654 - 1660
  • [7] Remifentanil Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Downregulating the NF-κB Signaling Pathway
    Ying Zhang
    Zhaohui Du
    Qing Zhou
    Yanlin Wang
    Jianguo Li
    [J]. Inflammation, 2014, 37 : 1654 - 1660
  • [8] Inhibition of PKR ameliorates lipopolysaccharide-induced acute lung injury by suppressing NF-κB pathway in mice
    Li, Yinjiao
    Xiao, Jinglei
    Tan, Yongchang
    Wang, Jun
    Zhang, Yan
    Deng, Xiaoming
    Luo, Yan
    [J]. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2017, 39 (04) : 165 - 172
  • [9] Nobiletin-Ameliorated Lipopolysaccharide-Induced Inflammation in Acute Lung Injury by Suppression of NF-κB Pathway In Vivo and Vitro
    Weifeng Li
    Ruoqi Zhao
    Xiumei Wang
    Fang Liu
    Jinmeng Zhao
    Qing Yao
    Wenbing Zhi
    Zehong He
    Xiaofeng Niu
    [J]. Inflammation, 2018, 41 : 996 - 1007
  • [10] Nobiletin-Ameliorated Lipopolysaccharide-Induced Inflammation in Acute Lung Injury by Suppression of NF-κB Pathway In Vivo and Vitro
    Li, Weifeng
    Zhao, Ruoqi
    Wang, Xiumei
    Liu, Fang
    Zhao, Jinmeng
    Yao, Qing
    Zhi, Wenbing
    He, Zehong
    Niu, Xiaofeng
    [J]. INFLAMMATION, 2018, 41 (03) : 996 - 1007