Effect of additives in medium on in-vitro maturation of goat oocytes

Present study was carried out to find the effect of combining EGF with IGF, cysteine and sodium pyruvate singly as additive in a medium consisting of TCM-199 + 100 mu l/ml foetal bovine serum + 100 mu M/ml cysteamine + 1 mu g/ml 17 beta- Oestradiol + 5 mu g/ml pFSH + 5 mu g/ml oLH + 10 per cent follicular fluid and 10 per cent oestrous goat serum on in-vitro maturation (IVM) of caprine oocytes on incubation at 38.5 degrees C for 24 hours in a CO2 incubator maintaining 5 per cent CO2 under humidified condition. The additives comprised 10 ng/ml EGF + 50 ng/ml IGF-1, 10 ng/ml EGF + 600 mu M/ml cysteine and 10 ng/ml EGF + 0.2 mM/ml sodium pyruvate. The IVM rate of oocytes on the basis of cumulus cells expansion and nuclear maturation was found to be significantly higher (P<0.05) with EGF + IGF-1 (88.74 +/- 1.85% and 61.71 +/- 1.61%) than with EGF + sodium pyruvate (82.86 +/- 0.97% and 54.62 +/- 1.88%), EGF + cysteine (78.58 +/- 1.45% and 49.02 +/- 1.52%) and without additive (control) (75.27 +/- 1.58% and 43.03 +/- 1.48%).