Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes

被引：1
|
作者：
Liu, Jin [1 ,2 ,3 ]
Gu, Hongzhou [1 ,2 ,3 ]
机构：
[1] Fudan Univ, Shanghai Canc Ctr, Shanghai 200433, Peoples R China
[2] Fudan Univ, Shanghai Stomatol Hosp, Inst Biomed Sci, Shanghai Key Lab Med Epigenet, Shanghai 200433, Peoples R China
[3] Shanghai Pudong Hosp, Fudan Univ Pudong Med Ctr, Ctr Med Res & Innovat, Shanghai 201399, Peoples R China
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 02期
基金：
中国国家自然科学基金;
关键词：
atomic force microscopy (AFM); biotechnology and BIOENGINEERING; microbiology; molecular biology;
D O I：
10.1016/j.xpro.2021.100531
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence up to a few kilobases. Our protocol provides a convenient yet economical way to store the sequence information of target ssDNA on phages for selective mass production on demand. For complete details on the use and execution of this protocol, please refer to Jia et al. (2021).
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页数：29
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