Virtual screening for novel quorum sensing inhibitors to eradicate biofilm formation of Pseudomonas aeruginosa

被引:163
|
作者
Zeng, Zhirui [1 ,2 ]
Qian, Li [3 ]
Cao, Lixiang [1 ,2 ]
Tan, Hongming [1 ,2 ]
Huang, Yali [2 ]
Xue, Xiaoli [1 ,2 ]
Shen, Yong [3 ]
Zhou, Shining [1 ,2 ]
机构
[1] Zhongshan Univ, Sch Life Sci, Dept Biochem, Guangzhou 510275, Peoples R China
[2] Zhongshan Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
[3] Zhongshan Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Peoples R China
关键词
quorum sensing inhibitor; virtual screening; baicalein; peudomonus aeruginosa; biofilm; Traditional Chinese Medicine;
D O I
10.1007/s00253-008-1406-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The automated docking program DOCK 5.3.0 was applied to screening for quorum sensing inhibitors (QSIs) of Peudomonus aeruginosa from a database containing 51 active components of Traditional Chinese Medicines with antibacterial activity. Five potential QSIs were revealed by the computer-based virtual screening. The compounds 3, 4, 5, 6, 7 inhibit biofilm formation of P. aeruginosa at a concentration of 200 mu M. Compound 4 (baicalein) does not inhibit the growth of P. aeruginosa; however, it significantly inhibits biofilm formation of the bacteria at a lower concentration of 20 mu M and promoted proteolysis of the signal receptor TraR protein in Escherichia coli at 4-40 mM. Baicalein and ampicillin showed synergistic activity against P. aeruginosa. These results suggested that baicalein can interfere with quorum sensing system of P. aeruginosa and will be developed as antibacterial agent with novel target.
引用
收藏
页码:119 / 126
页数:8
相关论文
共 50 条
  • [1] Virtual screening for novel quorum sensing inhibitors to eradicate biofilm formation of Pseudomonas aeruginosa
    Zhirui Zeng
    Li Qian
    Lixiang Cao
    Hongming Tan
    Yali Huang
    Xiaoli Xue
    Yong Shen
    Shining Zhou
    [J]. Applied Microbiology and Biotechnology, 2008, 79 : 119 - 126
  • [2] Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm
    Ding, Xian
    Yin, Bo
    Qian, Li
    Zeng, Zhirui
    Yang, Zeliang
    Li, Huixian
    Lu, Yongjun
    Zhou, Shining
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (12) : 1827 - 1834
  • [3] Inhibition of quorum sensing-controlled biofilm formation in Pseudomonas aeruginosa by quorum-sensing inhibitors
    Kalaiarasan, Ellappan
    Kottha, Thirumalaswamy
    Harish, Belgode Narasimha
    Gnanasambandam, Vasuki
    Sali, Veeresh Kumar
    John, James
    [J]. MICROBIAL PATHOGENESIS, 2017, 111 : 99 - 107
  • [4] Cajaninstilbene acid analogues as novel quorum sensing and biofilm inhibitors of Pseudomonas aeruginosa
    Huang, Zhi-Xing
    Yu, Jia-Hui
    Xu, Xing-Jun
    Xu, Xiao-Fang
    Zeng, Ting
    Lin, Jing
    Chen, Wei-Min
    [J]. MICROBIAL PATHOGENESIS, 2020, 148
  • [5] Itaconimides as Novel Quorum Sensing Inhibitors of Pseudomonas aeruginosa
    Fong, July
    Mortensen, Kim T.
    Norskov, Amalie
    Qvortrup, Katrine
    Yang, Liang
    Tan, Choon Hong
    Nielsen, Thomas E.
    Givskov, Michael
    [J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2019, 8
  • [6] Inhibition of biofilm formation and quorum sensing by soy isoflavones in Pseudomonas aeruginosa
    Yin, Liqing
    Zhang, Yongzhu
    Azi, Fidelis
    Zhou, Jianzhong
    Liu, Xiaoli
    Dai, Yiqiang
    Wang, Zhe
    Dong, Mingsheng
    Xia, Xiudong
    [J]. FOOD CONTROL, 2022, 133
  • [7] The aggregate distribution of Pseudomonas aeruginosa on biochar facilitate quorum sensing and biofilm formation
    Yan, Huicong
    Liu, Congcong
    Yu, Wentao
    Zhu, Xiaomin
    Chen, Baoliang
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2023, 856
  • [8] Role of quorum sensing in UVA-induced biofilm formation in Pseudomonas aeruginosa
    Pezzoni, Magdalena
    Pizarro, Ramon A.
    Costa, Cristina S.
    [J]. MICROBIOLOGY-SGM, 2020, 166 (08): : 735 - 750
  • [9] A role for TonB1 in biofilm formation and quorum sensing in Pseudomonas aeruginosa
    Abbas, Abdelhamid
    Adams, Claire
    Scully, Norma
    Glennon, Jeremy
    O'Gara, Fergal
    [J]. FEMS MICROBIOLOGY LETTERS, 2007, 274 (02) : 269 - 278
  • [10] Nanoformulated meloxicam and rifampin: inhibiting quorum sensing and biofilm formation in Pseudomonas aeruginosa
    Khorramdel, Malihe
    Ghadikolaii, Fatemeh Peyravii
    Hashemy, Seyed, I
    Javid, Hossein
    Tabrizi, Masoud H.
    [J]. NANOMEDICINE, 2024, 19 (07) : 615 - 632