Itaconimides as Novel Quorum Sensing Inhibitors of Pseudomonas aeruginosa

被引:46
|
作者
Fong, July [1 ]
Mortensen, Kim T. [2 ]
Norskov, Amalie [2 ]
Qvortrup, Katrine [2 ]
Yang, Liang [1 ,3 ]
Tan, Choon Hong [1 ,4 ]
Nielsen, Thomas E. [1 ,5 ]
Givskov, Michael [1 ,5 ]
机构
[1] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore, Singapore
[2] Tech Univ Denmark, Dept Chem, Lyngby, Denmark
[3] Southern Univ Sci & Technol, Shenzhen, Peoples R China
[4] Nanyang Technol Univ, Sch Phys & Math Sci, Div Chem & Biol Chem, Singapore, Singapore
[5] Univ Copenhagen, Costerton Biofilm Ctr, Dept Immunol & Microbiol, Copenhagen, Denmark
基金
新加坡国家研究基金会;
关键词
quorum sensing; biofilm; itaconimides; antivirulence; chemical biology; CYSTIC-FIBROSIS; GENES; EXPRESSION; VIRULENCE; BIOFILMS; IDENTIFICATION; RESISTANCE; IMPACT; SUSCEPTIBILITY; INFECTION;
D O I
10.3389/fcimb.2018.00443
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Pseudomonas aeruginosa is known as an opportunistic pathogen that often causes persistent infections associated with high level of antibiotic-resistance and biofilms formation. Chemical interference with bacterial cell-to-cell communication, termed quorum sensing (QS), has been recognized as an attractive approach to control infections and address the drug resistance problems currently observed worldwide. Instead of imposing direct selective pressure on bacterial growth, the right bioactive compounds can preferentially block QS-based communication and attenuate cascades of bacterial gene expression and production of virulence factors, thus leading to reduced pathogenicity. Herein, we report on the potential of itaconimides as quorum sensing inhibitors (QSI) of P. aeruginosa. An initial hit was discovered in a screening program of an in-house compound collection, and subsequent structure-activity relationship (SAR) studies provided analogs that could reduce expression of central QS-regulated virulence factors (elastase, rhamnolipid, and pyocyanin), and also successfully lead to the eradication of P. aeruginosa biofilms in combination with tobramycin. Further studies on the cytotoxicity of compounds using murine macrophages indicated no toxicity at common working concentrations, thereby pointing to the potential of these small molecules as promising entities for antimicrobial drug development.
引用
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页数:11
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