Fruiting bodies of Hericium erinaceus (Bull.) Pers. - a new source of water-insoluble (1→3)-α-D-glucan

被引：3

作者：

Wiater, Adrian ^{[1
]}

Choma, Adam ^{[2
]}

Komaniecka, Iwona ^{[2
]}

Pleszczynska, Malgorzata ^{[1
]}

Siwulski, Marek ^{[3
]}

Polak, Paulina ^{[4
]}

Janusz, Grzegorz ^{[5
]}

Szczodrak, Janusz ^{[1
]}

机构：

[1] Marie Curie Sklodowska Univ, Dept Ind Microbiol, Akad 19, PL-20033 Lublin, Poland

[2] Marie Curie Sklodowska Univ, Dept Genet & Microbiol, Akad 19, PL-20033 Lublin, Poland

[3] Poznan Univ Life Sci, Dept Vegetable Crops, Dabrowskiego 159, PL-60594 Poznan, Poland

[4] Marie Curie Sklodowska Univ, Dept Ind Microbiol, Students Sci Assoc, Akad 19, PL-20033 Lublin, Poland

[5] Marie Curie Sklodowska Univ, Dept Biochem, Akad 19, PL-20033 Lublin, Poland

来源：

ACTA SOCIETATIS BOTANICORUM POLONIAE | 2016年 / 85卷 / 03期

关键词：

Hericium erinaceus; (1 -> 3)-alpha-D-glucan; cell wall; fruiting body; carbohydrate analysis; STRUCTURAL-CHARACTERIZATION; FOURIER-TRANSFORM; POLYSACCHARIDE; SPECTROMETRY; MYCELIA; GLUCANS;

D O I：

10.5586/asbp.3506

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A water-insoluble polysaccharide (WIP) was isolated from the fruiting bodies of Hericium erinaceus HE01 by an alkaline solution with the yield of 5%. Structural and compositional analyses by total acid hydrolysis, methylation analysis, FT-IR, FT-Raman, and H-1 NMR spectroscopy as well as other instrumental techniques showed predominantly glucose linked by alpha-glycosidic bonds and small amounts of mannose, xylose, rhamnose, galactose, and ribose. The methylation analysis showed that (1 -> 3)-linked Glcp is the major constituent (70.8%) of the polymer, while the 3,4 substituted D-Glcp represents the main branching residue of the glucan. The presence of (1 -> 3)-alpha-D-glucan in the hyphae of H. erinaceus was additionally confirmed by the use of specific fluorophore-labeled antibodies.

引用

页数：6