Multiple micro-contact printing of extra cellular matrix with fine alignment

This study performed multiple micro-contact printing of extra-cellular matrix with a fine alignment system for the position and the posture of polydimethylsiloxane (PDMS) stamp. The position and the posture were adjusted with a 4-axis automated stage with a resolution of 1 m or 0.0025 deg. The adjustment was achived by monitoring the surface shape of PDMS stamp measured by using a laser displacement sensor in the alignment system. After the adjustment, fluorescent-dye-conjugated fibronectin applied on the surface of PDMS stamp was printed onto a cell culture dish. As the result of multiple printing, two different patterns of fibronectin were located on the same dish within an error of 50 mu m.