Purification and characterization of N-acetyl-D-glucosamine transferase from pupae of Papilio xuthus Linne

被引：0
|
作者：
Maeda, R [1 ]
Nozawa, A [1 ]
Matsumoto, M [1 ]
Kondo, K [1 ]
机构：
[1] Doshisha Univ, Dept Chem Engn & Mat Sci, Kyoto 6100321, Japan
来源：
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 2005年 / 38卷 / 06期
关键词：
N-acetyl-D-glucosamine transferase; purification; chitooligosaccharide;
D O I：
10.1252/jcej.38.455
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
In this work three kinds of N-acetyl-D-glucosamine transferase were purified from the crude extract of pupae of Papilio, xuthus Linne through Sephadex G-25 gel filtration chromatography and CM-Sephadex C-50 ion exchange chromatography. Thermal stability of these enzymes were 35-55 degrees C and optimal pH for transferase activity appeared at 6.5-7.0. Each enzyme could react with N-acetyl-D-glucosamine, and produced insoluble deposit suggesting highly polymerized chitooligosaccharides. These catalytic properties somewhat differ from those of chitintransferase purified from pupae of Pieridae belonging to the same order.
引用
收藏
页码：455 / 457
页数：3
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