Haploids and doubled haploids in Brassica spp. for genetic and genomic research

被引：70
|
作者：
Ferrie, Alison M. R. [1 ]
Moellers, Christian [2 ]
机构：
[1] CNR, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
[2] Univ Gottingen, Dept Nutzpflanzenwissensch, Abt Pflanzenzuchtung, D-37075 Gottingen, Germany
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2011年 / 104卷 / 03期
关键词：
Doubled haploids; Breeding; Transformation; Mutagenesis; Genomics; MICROSPORE-DERIVED EMBRYOS; IN-VITRO SELECTION; MOLECULAR MARKERS; OIL CONTENT; NAPUS L; IMPROVED RESISTANCE; SUBSTITUTION LINES; LINKAGE ANALYSIS; ACID CONTENT; QTL ANALYSIS;
D O I：
10.1007/s11240-010-9831-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.
引用
收藏
页码：375 / 386
页数：12
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