Label-Free Detection of DNA Hybridization Using Surface Enhanced Raman Spectroscopy

被引：224

作者：

Barhoumi, Aoune ^{[1
]}

Halas, Naomi J. ^{[1
,2
,3
]}

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77005 USA

[2] Rice Univ, Dept Elect & Comp Engn, Houston, TX 77005 USA

[3] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 37期

关键词：

NANOPARTICLE PROBES; SCATTERING; 2-AMINOPURINE; MICROARRAYS; CHIPS;

D O I：

10.1021/ja105678z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The SERS spectrum of DNA is strongly dominated by the strong spectral feature of adenine at 736 cm(-1); the presence of adenine can serve as an endogenous marker for the label-free SERS-based detection of DNA hybridization when the probe DNA sequence is adenine-free. The substitution of 2-aminopurine for adenine on the probe DNA sequence enables the detection of a target sequence using SERS, upon hybridization of the target with the 2-AP-substituted probe DNA sequence.

引用

页码：12792 / 12793

页数：2

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