Effects of chlorpyrifos on reproductive performances of guppy (Poecilia reticulata)

Guppy (Poecilia reticulata) was selected to investigate the effects of chlorpyrifos on reproductive performances. Male and female guppy with proven fertility were selected from our own colony and the groups of fish (n = 72/group) were exposed to pre-determined chlorpyrifos concentrations (0.002 mug/l, 2 mug/l) based on the 96-h LC50 for guppy. Mating behavior of males was recorded on the 2nd day of exposure. Offspring were counted and survival recorded on the 14th day. Gonopodial thrusts (8/15 min) in 0.002 mug/l and (4/15 min) in 2 mug/l were significantly different from the control group (11/15 min). Similarly, live birth reduced significantly to 8/female in 2 mug/l compared to 27/female in the control group. Survival of offspring after 14 days was reduced to 47% in the 2 mug/l group compared to 94% of survival in the control. Our findings demonstrate that low soluble concentrations of chlorpyrifos affect mating behavior, number of offspring and offspring survival of guppy. (C) 2004 Elsevier Ltd. All rights reserved.