Analysis of Leuconostoc citreum strains using multilocus sequence typing

被引：7

作者：

Sharma, Anshul ^{[1
]}

Kaur, Jasmine ^{[1
]}

Lee, Sulhee ^{[1
]}

Park, Young-Seo ^{[1
]}

机构：

[1] Gachon Univ, Dept Food Sci & Biotechnol, Seongnam 13120, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2018年 / 27卷 / 06期

关键词：

Lactic acid bacteria; Leuconostoc citreum; MLST; Sequence type; Alleles; IDENTIFICATION; FERMENTATION; KIMCHI;

D O I：

10.1007/s10068-018-0417-y

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objective of this study was to perform genetic diversity analysis of 13 strains isolated from South Korean foods by multilocus sequence typing (MLST). For typing, seven housekeeping loci (atpA, dnaA, dnaK, gyrB, pheS, pyrG, and rpoA) were selected, amplified and analyzed. Fifty-one polymorphic sites varying from 1 to 22 in each species were identified. Thirteen sequence types were generated with allele numbers ranged from 2 to 10. The overall relationship between strains was assessed by unweighted pair group method with arithmetic mean dendrogram and minimum spanning tree. In addition, combined spits tree analysis revealed intragenic recombination. No clear relationship was observed between the isolation sources and strains. The developed MLST scheme enhanced our knowledge of the population diversity of Leu. citreum strains and will be used further for the selection of industrially important strain.

引用

页码：1755 / 1760

页数：6

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