Facilitating Subtiligase-Catalyzed Peptide Ligation Reactions by Using Peptide Thioester Substrates

被引：15
|
作者：
Tan, Xiaohong [1 ,2 ]
Yang, Renliang [3 ]
Liu, Chuan-Fa [3 ]
机构：
[1] Carnegie Mellon Univ, Dept Chem, 4400 5th Ave, Pittsburgh, PA 15213 USA
[2] Carnegie Mellon Univ, Ctr Nucle Acids Sci & Technol, Pittsburgh, PA 15213 USA
[3] Nanyang Technol Univ, Sch Biol Sci, Div Chem Biol & Biotechnol, Singapore 637551, Singapore
来源：
ORGANIC LETTERS | 2018年 / 20卷 / 21期
关键词：
NATIVE CHEMICAL LIGATION; SOLID-PHASE SYNTHESIS; UNPROTECTED PEPTIDES; PROTEIN-SYNTHESIS; THIOACIDS; LIGASE; SCOPE;
D O I：
10.1021/acs.orglett.8b02747
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
By facilitating formation of the aryl-enzyme intermediate, peptide thioesters can largely promote subtiligase-catalyzed ligation reactions over their ester counterparts. This offers a significant addition to the repertoire of enzymatic methods for protein chemical synthesis and modification.
引用
收藏
页码：6691 / 6694
页数：4
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