Analysis of nutrient-dependent transcript variations in Neurospora crassa

被引：10

作者：

Aign, V ^{[1
]}

Hoheisel, JD ^{[1
]}

机构：

[1] Deutsch Krebsforschungszentrum, Div Funct Genome Anal, D-69120 Heidelberg, Germany

来源：

FUNGAL GENETICS AND BIOLOGY | 2003年 / 40卷 / 03期

关键词：

DNA-microarray analysis; transcriptional profiling; correspondence analysis; acetate metabolism; Neurospora crassa;

D O I：

10.1016/S1087-1845(03)00106-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Nutrient-dependent variations in transcript levels of the filamentous fungus Neurospora crassa were studied on a microarray containing some 4700 cDNAs. Cells were grown in minimal and acetate medium. The isolated RNA was analyzed in comparison to the results obtained upon the hybridization of samples prepared from the RNA of cells grown in full medium. Altogether, 160 cDNA clones exhibited significant variations, falling into five distinct subgroups of very similar transcription profiles. This is indicative of the occurrence of a high degree of co-regulation of genes in N. crassa. Especially the regulation of the expression of proteins involved in metabolic pathways was found to be strongly regulated at the RNA level. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：225 / 233

页数：9

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