Obtaining transgenic bovine skin fibroblasts containing human interferon alpha gene

被引：0

作者：

Lipinski, Daniel ^{[2
,3
]}

Duszewska, Anna Maria ^{[1
]}

Zeyland, Joanna ^{[2
]}

Maly, Ewa ^{[3
]}

Gawron, Witold ^{[1
]}

Rynkowska, Anna ^{[1
]}

Reklewski, Zygmunt ^{[1
]}

Slomski, Ryszard ^{[2
,3
]}

机构：

[1] Polish Acad Sci, Inst Genet & Anim Breeding, PL-05552 Wolka Kosowska, Poland

[2] Acad Agr Poznan, Dept Biochem & Biotechnol, PL-60637 Poznan, Poland

[3] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland

来源：

ANIMAL SCIENCE PAPERS AND REPORTS | 2007年 / 25卷 / 04期

关键词：

cattle; fibroblasts; interferon; lipofection; skin; transgenesis;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The study aimed at obtaining the heifer's transgenic cell lines with integrated human interferon alpha gene (IFN alpha) having in view cloning of Polish Red cattle. The gene construct used (pbLGIFN-GFPBsd) contained the entire human interferon alpha gene under the bovine beta-lactoglobulin promoter (bLG) and the green fluorescent protein gene (gfp) fused to the blasticidin resistance gene (bsd) under the human cytomegalovirus (CMV) immediate-early promoter. The gene construct was introduced by lipofection into heifer's ear fibroblasts. The GFP- and Bsd-positive cell lines have been obtained. The molecular analysis (PCR) and cytogenetic characterization (FISH) confirmed obtaining the stable heifer's transgenic cell lines with integrated human IFN alpha gene.

引用

页码：211 / 220

页数：10

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