Activated Platelet-Rich Plasma Improves Fat Graft Survival in Nude Mice: A Pilot Study

BACKGROUND Numerous processing methods have been proposed to enhance the survival of fat grafts, but no definitive treatment protocol is available. Platelet-rich plasma (PRP) contains a high concentration of platelets with neovascularization properties and has the potential to promote fat graft survival. OBJECTIVE To evaluate the effect of activated PRP on human graft quality and survival. MATERIALS AND METHODS Using the scalp model, nude mice received fat mixed with PRP followed by injection of thrombin and calcium chloride to activate the PRP (PRP group) or fat mixed with saline (control group). After 10 weeks, mice were killed, the volume and weight of the fat graft were determined, and graft sections were examined using light microscopy. RESULTS Fat graft volume and weight were significantly higher in the PRP group than in the control group, and histologic evaluation revealed greater vascularity, fewer cysts and vacuoles, and less fibrosis in the PRP group than in the control group. The cellular integrity and inflammation were not statistically different between the two groups. CONCLUSION PRP treatment improved the survival and quality of fat grafts. Safer methods of PRP activation and preparation should be further investigated for potential application in humans. The authors have indicated no significant interest with commercial supporters.