Gene expression profile in liver of hB1F transgenic mice

被引：0
|
作者：
Wang, Shui-Liang [1 ,2 ]
Yang, Hua [1 ]
Xie, You-Hua [3 ]
Wang, Yuan [3 ]
Li, Jian-Zhong [1 ]
Wang, Long [4 ]
Wang, Zhu-Gang [4 ]
Fu, Ji-Liang [1 ,4 ]
机构：
[1] Second Mil Med Univ, Dept Med Genet, Shanghai 200433, Peoples R China
[2] Fuzhou Gen Hosp, PLA Certer Lab Med, Fuzhou 350025, Fujian Province, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China
[4] Shanghai Nanfang Res Ctr Model Organisms, Shanghai 201203, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2004年 / 10卷 / 20期
基金：
中国国家自然科学基金;
关键词：
D O I：
暂无
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice. METHODS: Transgene expression was analyzed with RTPCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of the differentially expressed genes were verified with RT-PCR. RESULTS: The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes. CONCLUSION: The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.
引用
收藏
页码：3006 / 3010
页数：5
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